PLEUROPNEUMONIA-LIKE (MYCOPLASMA) INFECTIONS OF TISSUE CULTURE

Elimination of pleuropneumonia-like organisms from tissue culture.

Pleuropneumonia-like organisms (PPLO) are the crabgrass of tissue culture. Many reports exist in the literature of successful attempts to eliminate these organisms from tissue cultures, but usually such reports were stimulated by the failure of some previously described method to be successful in the hands of the investigator. Although our cultures exhibited no overt manifestations of contamina...

Culture of Human Genital "t-strain" Pleuropneumonia-like Organisms.

Ford, Denys K. (University of British Columbia, Vancouver, Canada). Culture of human genital "T-strain" pleuropneumonia-like organisms. J. Bacteriol. 84:1028-1034. 1962.-The conditions under which "T-strain" pleuropneumonia-like organisms, as described by Shepard, are best cultured were investigated. The organisms were found to grow on several types of nutrient agar and broth, of which PPLO med...

Antibiotic control of Mycoplasma in tissue culture.

Seven of eight strains of Mycoplasma (PPLO) were found to be sensitive to the deoxystreptamines, certain macrolides, and the tetracyclines. These antibiotics are relative noncytotoxic. Kanamycin and tetracycline were useful in eliminating PPLO (pleuropneumonia-like organisms) strain Squibb no. 1 from a HeLa cell line which was deliberately contaminated with PPLO. Repeated exposure of M. laidlaw...

[Contamination of tissue cultures with pleuropneumonia-like organisms].

Enumeration of pleuropneumonia-like cultures.

Kelley and Morton (J. Bacteriol., 67, 129, 1954) and Smith (Appl. Microbiol., 4, 254, 1956) observed that use of colony counts or turbidimetric methods of counting pleuropneumonialike organisms are difficult at best. In some studies on the limitations of "coding" of DNA (Morowitz and Cleverdon, Biochim. et Biophys. Acta, 34, 578, 1959), we repeatedly used the Bausch and Lomb Spectronic 20 at 62...